Revealing real-time 3D in vivo pathogen dynamics in plants by

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Abstract

This paper presents a novel method for real-time 3D in vivo visualization of pathogen dynamics in plants using label-free optical coherence tomography (OCT). The technique leverages intrinsic signal fluctuations as tissue contrast to image filamentous pathogens and nematodes without the need for invasive histological techniques or transgenic pathogens. The authors analyze 3D images of lettuce downy mildew infection to quantify hyphal volume and length, demonstrating the potential for digital phenotyping and studying pathogen vitality.

Publisher

Nature Communications

Published On

Sep 27, 2024

Authors

Jos de Wit, Sebastian Tonn, Mon-Ray Shao, Guido Van den Ackerveken, Jeroen Kalkman

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