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Abstract
This study aimed to improve the miRNA isolation process from cultured cells using a commercially available extraction kit. The researchers investigated factors influencing RNA adsorption (ethanol concentration, Ca²⁺ addition) and elution (temperature, pH). Optimal conditions were determined as 65% (v/v) ethanol for adsorption and TE buffer (pH 8.0) at 55°C for elution. The modified method increased endogenous miR-21 recovery approximately sixfold compared to the original kit.
Publisher
Scientific Reports
Published On
Dec 03, 2020
Authors
Wen-Pin Hu, Yu-Chi Chen, Wen-Yih Chen
Tags
miRNA isolation
RNA adsorption
extraction kit
ethanol concentration
optimal conditions
miR-21 recovery
elution

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