Exosome based analysis for Space Associated Neuro-Ocular Syndrome and health risks in space exploration

Molecular profiling to characterize the effects of environmental exposures is important from the human health and performance as well as the occupational medicine perspective in space exploration. We have developed a novel exosome-based platform that allows profiling of biological processes in the body from a variety of body fluids. The technology is suitable for diagnostic applications as well as studying the pathophysiology of the Space Associated Neuro-Ocular Syndrome in astronauts and monitoring patients with chronically impaired cerebrospinal fluid drainage or elevated intracranial pressure. In this proof-of-concept, we demonstrate that: (a) exosomes from different biofluids contain a specific population of RNA transcripts; (b) urine collection hardware aboard the ISS is compatible with exosome gene expression technology; (c) cDNA libraries from exosomal RNA can be stored in dry form and at room temperature, representing an interesting option for the creation of longitudinal molecular catalogs that can be stored as a repository for retrospective analysis.

Sudipto K. Chakrabortty, Yevgenia L. Khodor, Robert R. Kitchen, Dulaney L. Miller, Kailey M. Babcock, Kyle S. Manning, Steven P. Lang, Vasisht Tadigotla, Wei Yu, Eric Bershad, Johan Skog, Susana Zanello