This paper introduces a dynamic DNA-based information storage system called DORIS (Dynamic Operations and Reusable Information Storage). DORIS utilizes a simple architecture consisting of a T7 promoter and a single-stranded overhang domain (ss-dsDNA) to enable dynamic access and manipulation of stored information. The overhang serves as a physical address for accessing specific DNA strands, while the T7 promoter allows for repeated information access through transcription without destroying the DNA. The study demonstrates the scalability, increased storage density, and capacity of DORIS compared to PCR-based methods, along with its capability for in-storage file operations such as locking, unlocking, renaming, and deleting.

Kevin N. Lin, Kevin Volkel, James M. Tuck, Albert J. Keung