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Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction

Biology

Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction

Y. Kuritani, K. Sato, et al.

This research paper reveals the intricate metabolic pathway from levoglucosan to glucose in *Bacillus smithii* S-2701M, highlighting the role of four key enzymes in the process. Researchers Yuya Kuritani, Kohei Sato, Hideo Dohra, Seiichiro Umemura, Motomitsu Kitaoka, Shinya Fushinobu, and Nobuyuki Yoshida shed light on its significance for biofuel production and biomass utilization.

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Playback language: English
Abstract
This research paper elucidates the complete metabolic pathway of levoglucosan (LG) to glucose in *Bacillus smithii* S-2701M, involving four enzymes: LG dehydrogenase (LgdA), LgdB1, LgdB2, and LgdC. The study demonstrates that these enzymes sequentially catalyze 3-dehydrogenation, β-elimination, hydration, and reduction of LG, respectively, resulting in glucose formation. This pathway resembles that of glycoside hydrolase family 4 enzymes. The findings suggest potential applications in biofuel production and utilization of lignocellulosic biomass.
Publisher
Scientific Reports
Published On
Nov 18, 2020
Authors
Yuya Kuritani, Kohei Sato, Hideo Dohra, Seiichiro Umemura, Motomitsu Kitaoka, Shinya Fushinobu, Nobuyuki Yoshida
Tags
levoglucosan
glucose
Bacillus smithii
metabolic pathway
biofuel production
lignocellulosic biomass
enzymes
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